大鼠2型糖尿病早期肾小管细胞聚核糖性死亡

doi:10.3969/j.issn.0529-1356.2013.03.023

解剖学报 ›› 2013, Vol. 44 ›› Issue (3 ): 409-412.doi: 10.3969/j.issn.0529-1356.2013.03.023

• 组织学胚胎学发育生物学 • 上一篇下一篇

大鼠2型糖尿病早期肾小管细胞聚核糖性死亡

李淑玲¹阎文柱^2*

1.辽宁医学院组织学胚胎学教研室，辽宁锦州 121001; 2. 辽宁医学院解剖学教研室，辽宁锦州 121001

收稿日期:2012-11-12 修回日期:2013-01-21 出版日期:2013-06-06 发布日期:2013-07-16
通讯作者: 阎文柱 E-mail:ywz2468@sina.com
基金资助:
辽宁省科技厅计划项目2012225019;其他(不属于以上基金类别的请自行输入下框)

Parthanatos involved in tubular damages of the kidney in rats with type 2 diabetics

LI Shu-ling¹ YAN Wen-zhu ^2*

1.Department of Histology and Embryology，Liaoning Medical University, Liaoning Jinzhou 121001, China;2.Department of Anatomy, Liaoning Medical University, Liaoning Jinzhou 121001, China

Received:2012-11-12 Revised:2013-01-21 Online:2013-06-06 Published:2013-07-16

摘要/Abstract

摘要：

目的探讨2型糖尿病大鼠肾小管细胞聚核糖性死亡的形态学特点。方法 20只雄性SD大鼠分为正常组和2型糖尿病组，在光学显微镜和
电子显微镜下观察两组大鼠肾小管细胞结构的变化，用免疫组织化学染色、免疫印迹方法检测多聚二磷酸腺苷核糖聚合酶-1（PARP-1）蛋白的
表达。结果 2型糖尿病早期肾小管细胞内有大量糖原聚集，细胞肿胀，部分细胞坏死，主要为空泡样坏死。免疫组织化学染色显示，2型糖尿病
肾小管上皮细胞PARP-1表达阳性。免疫印迹法显示2型糖尿病肾小管细胞中PARP-1蛋白表达增强。结论 2型糖尿病早期肾小管细胞发生了坏死，
聚核糖性死亡（PARP-1介导的细胞死亡）参与了2型糖尿病早期肾小管细胞的损伤。

关键词: 2型糖尿病, 肾小管损伤, 坏死, 聚核糖性死亡, 免疫组织化学, 免疫印迹法, 大鼠

Abstract:

Objective To observe the parthanatos involved in tubular cell damages of kidney in rats with type 2 diabetics.
Methods Animal models of type 2 diabetics were established. Light microscopy, electron microscopy, immunohistochemistry and
Western blotting were used in this study. Results A large number of glycogen accumulation, cell swelling, and partial necrosis,
mainly for the vacuolar necrosis were seen in early type 2 diabetes renal tubular cells. Immunohistochemical staining of PARP-1
showed that the positive cells were found in epithelium of renal tubules of type 2 diabetic kidney. Compared to the control
kidney, PARP-1 expressed much stronger by immunoblot analysis. Conclusion Necrosis and parthanatos are involved in renal tubule
damages induced by type 2 diabetes in rats.

Key words: Type 2 diabetes mellitus, Renal tubule damage, Necrosis, Parthanatos, Immunohistochemistry, Western blotting, Rat

李淑玲阎文柱* . 大鼠2型糖尿病早期肾小管细胞聚核糖性死亡[J]. 解剖学报, 2013, 44(3 ): 409-412.

LI Shu-ling YAN Wen-zhu*. Parthanatos involved in tubular damages of the kidney in rats with type 2 diabetics[J]. AAS, 2013, 44(3 ): 409-412.

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大鼠2型糖尿病早期肾小管细胞聚核糖性死亡

Parthanatos involved in tubular damages of the kidney in rats with type 2 diabetics

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